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KMID : 0360419940300010087
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Korean Journal of Pharmacology
1994 Volume.30 No. 1 p.87 ~ p.100
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Interrelationship between Dopaminergic Receptors and Catecholamine Secretion from the Rat Adrenal Gland
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Lim Dong-Yoon
Yoon Joong-Keun
Moon Baek
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Abstract
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It has been known for some time that dopamine-containing cells are existed in sympathetic ganglia, i.e., small, intensely fluorescent cells. However, its role and mechanism of action as a peripheral neurotransmitter are poorly understood so far. In the present study, an attempt was made to examine the effect of apomorphine, which is known to be a selective agonist of dopaminergic . receptor on secretion of catecholamines (CA) from the isolated perfused rat adrenal gland. The perfusion of a low concentration of 10uM apomorphine into an adrenal vein for 20 min produced significant reduction in CA secretion induced by 5.32 mM ACh, 56 mM KCl, 100 uM DMPP and 100 uM McN-A-343. Increasing apomorphine concentration to 30 uM led to more markedly decreased CA secretion as compared to the case of 10 uM apomorphine and also did inhibit clearly CA release by Bay-K-8644. Furthermore, in adrenal glands preloaded with a higher dose of 100 uM apomorphine, CA releases evoked by ACh, excess , DMPP and McN-A-343 were almost abolished by the drug. The perfusion of metoclopramide, which is well-known as a selective dopaminergic antagonist, produced significantly inhibitory effect of CA release by ACh, DMPP and McN-A-343 but did not affect that by excess . However, preloading of 30uM apomorphine in the presence of metoclopramide did not modify the CA secretory effect of excess and DMPP. These experimental results demonstrate that apomorphine causes dose-dependent inhibition of CA secretion by cholinergic receptor stimulation and also by membrane depolarization from the isolated perfused rat adrenal gland, suggesting that these effects appear to be exerted by inhibiting influx of extracellular calcium into the rat adrenal medullary chromaffin cells through activation of inhibitory dopaminergic receptors.
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KEYWORD
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Dopaminergic, Catecholamine secretion, Adrenal gland
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